Chapter 13: Single cell metabolomics
by Minakshi Prasad, Mayukh Ghosh, Rajesh Kumar
Single-cell techniques are evolving rapidly to capture the stimuli-responsive deterministic variations occurring at the cellular and sub-cellular level, precisely targeting the heterogeneity of spatiotemporal analyte expression arising from divergent cellular phenotypes. Single-cell metabolomics (SCM) is relatively nascent than the other single-cell techniques such as single-cell sequencing and single-cell proteomics, yet the potential of SCM has already been perceived through diverse biological applications including but not limited to microbial technology, plant science and agriculture, developmental biology, aging and senescence study, stem cell biology, functional genomics, nutrition research, environmental biology, system biology, immunology, drug discovery and toxicity analyses, disease diagnosis and therapy, etc. Currently bulk of the SCM research has been directed towards establishment and validation of the newer SCM analytical modalities along with proof-of-concept interventions. Now the time has come to channelize the efforts towards improving the frequency of ‘bench to beside’ transition of these state-of-the-art SCM techniques for commonplace biological applications from the on-going status of typically specialized interventions. Standardization of SCM procedures and customization of general SCM workflow compatible with broader samples and analytes, strengthening of SCM database, facilitation of data sharing option from different analytical platforms and among diverse scientific beneficiaries, integration of multiple single-cell intervention outcomes to generate reliable models for diagnostic and therapeutic intervention, etc. are certain measures which can improve the on-field transition of SCM interventions for diverse biological applications.
Dr. Minakshi is former Professor & Head of Department of Animal Biotechnology, LLR University of Veterinary and Animal Sciences (LUVAS), India. She was designated as Visiting Expert (2019) by The World Academy of Sciences. She was visiting scientist at London School of Hygiene and Tropical Medicine, London, UK and visiting scientist at University of Minnesota, USA. Her research interest is in the field of Bioinformatics and Biotechnology with specialization in animal viruses; molecular characterization, diagnosis and vaccine design. She has been conferred with various prestigious awards notably, International Women Day Award 2021, Lifetime Achievement Award 2019 by PISRF(I) in Thailand; International Research Ratna Award, Malaysia by “World Research Council” &“United Medical Council”, Award for Excellence in Research, ISVIB Woman Scientist Award, Designated Ambassador of the Vet2011 project by Executive Council, Ecole Nationale Vétérinaire de Lyon; ICAR-Jawaharlal Nehru Award, Prof. R.M. Sharma Memorial & J.V. Bhatt Awards Association of Microbiologists of India. She has been conferred with Fellowships (F) of prestigious International and National Academies including F-National Academy of Agricultural Sciences; F-National Academy of Veterinary Sciences; F-International Academy of Biosciences; F-International Science Congress Association; Fellow-Society for Applied Biotechnology; Commonwealth Academic Staff Fellowship. She is Review Editor of Frontiers in Microbiology. She has been keynote speaker/lead speaker/Chairperson/invited speaker in several National/International conference/symposia. She has published more than 150 research articles on veterinary microbiology and animal biotechnology in the peer-reviewed journals and authored/co-authored books and book chapters. She is a member of many international and national scientific societies and organizations.
He is actively engaged in teaching and research activity as Assistant Professor, Department of Veterinary Physiology and Biochemistry, RGSC, Banaras Hindu University, UP, India. His research interest is in Proteomics and Metabolomics, Nanotechnology, Reproduction, Molecular Parasitology, Antioxidant and Redox Biology with specialization in mass spectrometry-based proteomic analysis, immunoinformatics, NMR-based metabolomics analyses, Biochemical determinants and antioxidant analyses. He has been awarded with several prestigious fellowships and worked as referee for a number of prestigious International publication groups.
He has also authored or co-authored about 35 publications in the peer-reviewed international journals and several books and book chapters. He is also involved in the research work on urinary metabolomics-based pregnancy diagnosis in buffaloes for which patent has been filed. He is also actively involved as member of different national and international scientific societies and organizations.
He is working as scientist in department of Veterinary Physiology & biochemistry, Lala Lajpat Rai University of Veterinary & Animal Sciences (LUVAS), Hisar, India. He posses more than 8 years of experience in field of molecular diagnostics. He has authored more than 35 international publications in various journals. To his credit currently two patents and is primarily involved in experimentation related to livestock physiology and medicine. His are of interest includes omics platforms, blockchain and automation technology in animal husbandry. He primarily worked in area of reproductive physiology and pathology. To his credit he had discovered in-vitro method of early postpartum anestrous marker and early pregnancy diagnosis kit by using urine.